FastQCFastQC Report
Sun 14 Apr 2019
SRR6576500_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576500_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276548
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5740.20755890478325645No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.12511390427701521No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3350.12113629460346848No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2920.10558745678869491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8250.045.0812721
TACATGG19350.041.286192
GTACATG19450.041.1483461
ACATGGG19700.039.8370443
CATGGGG6800.036.6272054
ATCAACG10200.035.9361273
TCAACGC10400.035.245054
CAACGCA10500.034.909385
AACGCAG10650.034.858956
CATGGGT4400.033.1089824
ATGGGAG4850.032.943835
TATTAGA902.5893434E-531.3289322
GGGTACC1950.031.328937
TATCAAC11850.030.9323642
CATGGGA11000.030.7593154
ACGCAGA12300.030.182757
CGCAGAG12500.029.7213298
ATGGGTA2700.029.5884365
GTACTAG650.00609624428.9714131
GGTACCT1951.8189894E-1128.9399498
Produced by FastQC (version 0.11.2)