FastQCFastQC Report
Sun 14 Apr 2019
SRR6576497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437468
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11390.2603619007561696No Hit
CCTATAAGCAGTTCTTGTATTTTTATTCACACAGGTCTGATTAAGATGAA5870.13418124297091444No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5630.12869512741503378No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5550.12686642222974023No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5380.12298042371099144No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5320.12160889482202128No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGTG200.002162227470.49885
GTATCAA7950.047.3056031
ACATGGG25800.042.4450153
GTACATG26250.042.264181
TACATGG26350.041.925382
GGTACGT450.001012211741.786621
GTAATAC802.454235E-741.1243023
ATGGGTA4600.038.8254245
GTTAGGT500.001699107937.6079562
ATCAACG10550.036.5301863
GGTATCA3850.035.4100881
TCAACGC10900.035.3571974
CATGGGG13500.034.466084
CAACGCA11350.033.955375
AACGCAG11450.033.6588136
GTCTAAA600.00415388631.3399621
ATGGGAC2800.030.213775
TATCAAC13050.029.8990462
CATGGGA9200.029.6299294
CGTACAC650.00615057128.9225853
Produced by FastQC (version 0.11.2)