FastQCFastQC Report
Sun 14 Apr 2019
SRR6576496_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576496_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343720
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5690.16554172000465495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.11579192365879204No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG250.005222864456.4274259
GTACATG22300.036.142071
TACATGG21950.035.9744452
ACATGGG22250.034.2169383
GTATTAC702.1460014E-433.6661721
GGTACCT2900.032.4295588
GTATCAA10550.031.7195991
ATGGGTA3000.031.3303265
TAGACTG1351.3227691E-831.3303265
CATGGGG8250.030.1910424
GTACTTT9950.029.842781
GGGTACC3000.029.7638117
AACGCAG11350.028.1558886
TGGGTAC3350.028.0570096
ACTTTTT10650.027.8001483
GTACTAG856.695045E-427.7250841
GTCTTAC856.695045E-427.7250841
ATAATAT1205.8319183E-627.4140363
CATGGGA12350.027.398184
CATGGGT4850.027.1314184
Produced by FastQC (version 0.11.2)