FastQCFastQC Report
Sun 14 Apr 2019
SRR6576496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343720
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5000.14546724077737694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA250.005238989456.38354
ACACCGT405.679319E-446.986258
TACATGG22800.038.5537952
GTACATG23250.038.414131
ACATGGG23050.037.9151543
CATGGGG7450.037.2105874
TGCCCGC801.1551494E-535.2550741
CATGGGT5400.032.1942834
ATGGGTA2800.031.883535
GGGTACC2550.031.324177
AAGGCGT753.2764187E-431.3241676
GGTACCT2700.029.5839378
ATCGACC404.7870996E-429.36640716-17
GTATAAT1802.3646862E-1028.7263581
GTCTATA856.8005687E-427.651041
CATGGGA14300.027.271744
GTATCAA10750.027.110881
GTCTAGA1057.399444E-526.8610121
TGGGTAC3200.026.4297666
GTTCTAC909.4960706E-426.1148721
Produced by FastQC (version 0.11.2)