FastQCFastQC Report
Sun 14 Apr 2019
SRR6576495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376109
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5190.13799191191915108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG205.6694483E-447.01010580-81
GTATCAA10150.038.910091
ACATGGG22050.037.9391143
GTACATG22350.037.655161
TACATGG22500.036.9862022
AATTCGC352.1815303E-433.57864878-79
ATCAACG11700.032.5368043
TCAACGC12050.031.9817734
CAACGCA12200.031.5885545
CGCGGAA300.00415840531.33590582-83
AACGCAG12450.030.9542456
CATGGGA14150.030.8888874
TATCAAC13200.029.2071322
ATGGGAG5450.028.4572665
CATGGGT4700.027.9985754
CGCAGAG14650.026.9474338
ACGCAGA14550.026.809637
CATGGGG7500.026.318664
ATGGGAT3950.026.1758825
TGGGTAC2701.8189894E-1224.369136
Produced by FastQC (version 0.11.2)