FastQCFastQC Report
Sun 14 Apr 2019
SRR6576493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304491
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6200.20361849775527027No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3240.10640708592372188No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3100.10180924887763514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAG701.4115358E-953.729961
GTATCAA7450.042.911851
TACATGG16150.034.3505822
TCAACGC9350.034.1805654
CAACGCA9550.033.464745
ATCAACG9550.033.464743
CTAGTAC851.7510067E-533.175253
AACGCAG9650.033.1179586
GTACATG16800.033.021541
ACATGGG16800.032.7309463
ATAGACT1102.9598523E-629.9079934
TATCAAC11000.029.4904232
ACGCAGA11300.028.2821467
CTTTATA1503.668538E-828.2082332
GTATTAT856.7929004E-427.6551281
CGCAGAG12000.026.6323578
GGTATCA4250.026.5489231
AACGAAC909.503595E-426.11015394
ACTGAGC1859.978976E-925.4044748
CATGGGA8650.024.9933014
Produced by FastQC (version 0.11.2)