FastQCFastQC Report
Sun 14 Apr 2019
SRR6576491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653367
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13420.2053975790023065No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8570.13116671028686788No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7840.11999381664516268No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6580.10070909611290439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA200.002162923870.497029
GTATCAA17400.037.57891
CCGAACT1055.0671588E-835.8409231
AACGCAG20750.031.4830046
CAACGCA21200.030.8194485
TCAACGC21100.030.7427394
GTACATG34350.030.6760751
TACATGG33850.030.5592692
ATCAACG21700.029.8927123
GTCCTAA804.7541884E-429.4007571
GTCTATA1602.1100277E-929.4007571
CCCGCTA804.775553E-429.3782543
CCGCTAA650.00614892328.926284
CGCTAAA650.00614892328.926285
TTACACG650.00614892328.926283
ACATGGG35500.028.8651683
TTAGGTA1853.274181E-1027.9490414
TAGGTAT1704.0654413E-927.650125
GTATAGT1904.474714E-1027.2093738
GGTACCT6350.026.644548
Produced by FastQC (version 0.11.2)