FastQCFastQC Report
Sun 14 Apr 2019
SRR6576488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381985
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11860.31048339594486696No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6570.17199628257654095No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5670.1484351479770148No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5480.14346113067267038No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5140.1345602576017383No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4750.12435043260861028No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4610.12068536722646178No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4590.12016178645758342No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4580.11989999607314424No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4120.10785763838894197No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3870.10131287877796248No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3820.10000392685576658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9300.035.4173361
GTACATG19100.034.736541
GGGTATA550.002699372434.2214281
TACATGG20350.033.022012
ACATGGG20500.032.7803883
GTACATA600.00413403231.3696421
GTCTATA600.00413403231.3696421
AACGCAG11250.029.2400076
TGATATA650.00615365628.9186882
TCAACGC11500.028.6043534
CAACGCA11550.028.4805245
ATCAACG11750.027.995753
ATGGGAG5000.026.3160045
GTATAGG909.441946E-426.1413671
CATGGGG9950.025.9759564
CATGGGA10150.025.9270974
ACGCAGA13000.025.3038527
GAATAAC1501.1540615E-625.0792779
GTTCTAG950.001294224324.7655071
TATATAC950.00130414524.7330884
Produced by FastQC (version 0.11.2)