FastQCFastQC Report
Sun 14 Apr 2019
SRR6576486_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576486_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306393
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5750.18766747282085425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3570.11651702225573038No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3150.10280913728446799No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3070.10019811157565611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG19750.039.0276452
GTACATG19850.038.6700061
ACATGGG20500.036.676753
GTATCAA9500.034.6993681
ATGGGAG4350.032.4083375
CATGGGA11150.032.030484
CATGGGG6650.031.7991564
GGGTACC3200.030.8385567
ATGGGTA3700.030.481355
ATGGGGA3500.029.5378825
CTATACT804.7775035E-429.3700544
ATCAACG11000.029.0496523
TACCCCG650.0061522828.9182075
GGTACCT3350.028.0733018
AACGCAG10900.028.0228026
CATGGGT5550.027.941244
TCAACGC11300.027.8625624
CAACGCA11500.027.3779955
TGGGTAC3450.027.2417876
GCGCTCC700.0088052526.8701618
Produced by FastQC (version 0.11.2)