FastQCFastQC Report
Sun 14 Apr 2019
SRR6576486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306393
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5870.1915840113840721No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3280.1070520540612873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT4100.037.8190884
GTACATG20550.034.777341
TACATGG20700.034.752472
ATGGGTA2850.034.6222575
ATGACGG550.002717497334.172623
ATCATAC702.1847284E-433.5623933
GTATCAA10200.033.189211
ACATGGG21100.033.180643
TGGGTAC3300.031.32496
GGTACCT2800.028.5280348
TATACTA1005.320726E-528.192415
TAGTACA856.8153156E-427.6396184
CATGGGG7100.027.1335434
GGGTACC2950.027.0774577
GTAGGAC700.00883768326.8499153
ATCAACG12450.026.7960013
ATGGGAG4400.026.697365
TCAACGC12500.026.6888144
AACGCAG12500.026.3129166
CAACGCA12900.025.8612565
Produced by FastQC (version 0.11.2)