FastQCFastQC Report
Sun 14 Apr 2019
SRR6576485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342005
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5450.15935439540357596No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3640.10643119252642505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.10584640575430183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9200.045.0521051
TCAACGC11450.036.130254
AACGCAG11500.035.5643776
ATCAACG11650.035.106473
CAACGCA11700.034.9564365
GTACATG19000.033.713631
ATTACCT702.1789769E-433.5788463
TACATGG19500.032.545652
ACATGGG18650.032.26453
TATCAAC13950.029.3183022
ACGCAGA14050.029.101127
CGCAGAG13900.029.0770558
GGGTACC2650.028.3753287
ATGGGAC2500.028.206235
GTACTAG700.00873652126.9142441
ATGGGTA2450.026.8630775
TATAAGT700.00881799526.8630772
GGTACCT2850.026.3840778
CATGGGT4350.025.9367644
CATGGGA10650.025.60194
Produced by FastQC (version 0.11.2)