FastQCFastQC Report
Sun 14 Apr 2019
SRR6576485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342005
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6050.17689799856727242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7250.039.5488241
GTACATG18350.037.655031
TACACCG500.001700733337.5983055
TACATGG18650.037.049322
ACATGGG18950.035.4654163
GTACTAG702.1805117E-433.574821
ATGGGAG4350.032.412335
ATACCGT902.5894384E-531.331926
CATGGGA10500.030.8843214
AACGCAG9200.030.1399446
ATCAACG9150.029.7910043
CAACGCA9200.029.6290975
TCAACGC9200.029.6290974
ATGGGTA2550.029.4888655
TCCGACC804.775206E-429.3736748
ATAGACT1154.1865405E-628.6074054
GTATAAC1005.3090982E-528.202851
CATGGGG7350.028.1347854
GGTACCT2305.456968E-1226.5640188
CTAAGGC1258.0051905E-626.3188153
Produced by FastQC (version 0.11.2)