FastQCFastQC Report
Sun 14 Apr 2019
SRR6576484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181071
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3280.18114441296508No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2060.11376752765489781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG502.9992576E-547.0001371
TATACGG405.663897E-447.0001372
GTACATG12900.040.441981
ATGGGAG2600.039.758365
TACATGG13150.038.958292
ACATGGG13400.037.8702473
GTATCAA6650.037.4587551
CATGGGG3100.036.3771554
CATGGGA8000.035.2403684
ATAGGTA550.002714629534.17247894
ATAGCCT550.002714629534.1724784
ATATAAC550.002714629534.1724783
GGGTACC1256.2227627E-933.8307537
ATGGGGA2400.033.282575
AACGCAG7700.032.341816
CAACGCA7800.031.927175
ATCAACG7800.031.927173
GTGTAGA1201.6392369E-731.3334261
TCAACGC8150.031.1325954
TGGGTAC1401.877379E-830.206036
Produced by FastQC (version 0.11.2)