FastQCFastQC Report
Sun 14 Apr 2019
SRR6576482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293174
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9010.3073260248180261No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5310.18112110896600653No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5040.1719115610524808No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5040.1719115610524808No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4720.1609965413031169No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4610.15724450326427308No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4510.15383355959259687No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4070.13882540743722158No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3780.12893367078936058No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3670.12518163275051675No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3590.12245287781317579No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3560.12142959471167292No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3480.11870083977433196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370.11494880173548813No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3350.1142666130011529No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3280.11187895243097955No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3270.11153785806381195No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3070.10471597072045952No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3030.10335159325178904No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3030.10335159325178904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG301.3704621E-462.657452
AATAGTA408.02606E-658.7413675
TAGTATG408.02606E-658.7413677
CAATAGT352.9360256E-453.7063944
ATAGTAT451.6123791E-552.214556
ATAATAG601.6026388E-646.9930883
TAATAGT652.7806054E-643.3782424
GGGTACC3450.038.139327
TTACACT500.001701187737.5944754
TGGGTAC3650.037.3369756
GTACATG18800.036.7822381
TACATGG19050.036.509072
ATGGGAG3250.036.1485335
ACATGGG19350.035.7001763
GGGCTAT959.4296047E-734.626496
TTTAGGG550.002715679834.1767922
TAGGTAT1405.0749804E-1033.5664945
CTTAGGT1554.1836756E-1133.3499373
GGTACCT3950.033.33438
GTATCAA9500.033.176451
Produced by FastQC (version 0.11.2)