FastQCFastQC Report
Sun 14 Apr 2019
SRR6576481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331506
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9100.2745048355082563No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5370.16198801831641055No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5360.16168636465101688No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5020.15143014002763147No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4930.14871525703908828No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4930.14871525703908828No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4730.14268218373121452No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4460.13453753476558494No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4400.13272761277322281No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4030.12156642715365634No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3800.11462839284960152No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3730.11251681719184571No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3380.10195893890306662No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3320.10014901691070448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC503.0053561E-546.9984173
GTATCAA8650.046.8752251
GTACATG18550.038.1248661
AACGCAG10550.037.8660246
TACATGG18650.037.8003352
ACATGGG18900.037.300333
CAACGCA11150.035.828395
TCAACGC11250.035.5099144
ATCAACG11300.034.936883
CATGGGT4900.033.5702974
ATGGGTA3700.033.0259175
TATTATC3200.032.3114132
CATGGGG9500.031.6620924
GCTTATA600.00409336631.4318371
CGCAGAG12950.030.8483838
ACGCAGA13050.030.6119967
GGGTACC3850.030.5184547
GGTACCT3900.030.1271928
TTATCCT3750.030.0789854
TATCAAC13450.029.7016012
Produced by FastQC (version 0.11.2)