FastQCFastQC Report
Sun 14 Apr 2019
SRR6576481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331506
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11060.33362895392541914No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6330.19094677019420464No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5650.17043432094743383No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5200.15685990600471786No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5170.1559549450085368No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5070.1529384083545999No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4700.14177722273503346No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC4330.130616037115467No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4120.12428131014219954No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4020.12126477348826266No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3930.11854989049971947No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3790.11432673918420783No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3750.11312012452263306No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3660.11040524153408987No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3610.10889697320712144No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3550.1070870512147593No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC3540.10678539754936563No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3440.10376886089542874No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3380.10195893890306662No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3360.10135563157227924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9100.044.4302021
CTAGACA450.001012998941.777044
GTACATG19950.041.0041281
TACATGG19800.040.839882
ACATGGG20700.038.82543
TCAACGC10450.037.3294834
ATCAACG10650.037.0697673
AACGCAG10600.036.801246
CAACGCA10850.036.3864565
GGGTACC4050.033.653737
TGGGTAC3800.033.3941466
CATGGGG9550.031.9889644
CTAGCCT753.2708925E-431.332784
ATGGGTA4400.030.9767255
CGCAGAG12650.030.83748
TATCAAC12900.030.6133442
GGTACCT4300.030.6041138
ACGCAGA12800.030.4760257
CATGGGA8450.030.0349714
ATGGGAT1452.6484486E-829.1718985
Produced by FastQC (version 0.11.2)