FastQCFastQC Report
Sun 14 Apr 2019
SRR6576480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342196
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.1548819974517528No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4100.11981437538720499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7700.053.909061
ATCAACG9150.045.200363
TCAACGC9600.043.0815934
CAACGCA9650.042.8583685
AACGCAG10350.040.4138266
TACATGG16000.039.9542242
ACATGGG16700.037.711053
GTACATG16700.037.5668681
TATCAAC11700.034.952412
CGCAGAG12000.034.8569268
ACGCAGA12400.033.732517
CATGGGT3750.031.3320664
CATGGGG6100.030.8184244
ATGGGTA2650.030.1497275
GCAGAGT14350.028.8211359
CATGGGA9950.028.8129044
CCGTCGT1005.3142285E-528.198869
GGGTACC2700.027.8507257
GGTACCT2850.026.38498
GCCTATA909.4981096E-426.1138732
Produced by FastQC (version 0.11.2)