FastQCFastQC Report
Sun 14 Apr 2019
SRR6576480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342196
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3720.1087096284000982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.10812516803235574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT555.276887E-542.725086
GTATCAA7400.040.6703381
GTACATG15450.032.87191
TACATGG15050.032.4814872
ATCAACG9200.031.6722873
TCAACGC9500.030.6721124
ACATGGG16200.030.4614013
CAACGCA9600.030.3526085
GGTATCA4000.029.3906731
AACGCAG10300.028.2898126
ATATACT1555.0640665E-827.2889234
TATCAAC10700.027.2362292
ATATAGC1257.969311E-626.3340441
TATACAG1859.98989E-925.4041025
CATGGGA8950.025.205414
TGCCCGC950.001298669624.750041
ATGGGAG4000.024.6737355
CATGGGG7050.023.9987684
ACGCAGA12300.023.6898427
ACAAGTC1201.8350137E-423.4987933
Produced by FastQC (version 0.11.2)