FastQCFastQC Report
Sun 14 Apr 2019
SRR6576478_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576478_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337775
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5660.16756716749315373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.11190881503959738No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3380.10006661238990452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6350.044.565621
TACATGG17650.039.675122
ACATGGG17700.039.031993
GTACATG18600.038.2900921
CATGGGA9350.035.68814
CAACGCA8600.033.882075
AACGCAG8650.033.686226
ATCAACG8550.032.9808463
CATGGGT4750.032.651044
TGGGTAC2050.032.0959936
TCAACGC9050.031.678014
ATGGGTA3050.030.8181675
ATAACGC804.775185E-429.3735663
TATCAAC10200.028.106472
CATGGGG6300.027.6018264
ACGCAGA10650.027.3601657
ATGGGAG5200.027.1140615
GGTATGC700.00867747726.951591
GGGTACC2500.026.3187147
CGCAGAG11300.025.7863528
Produced by FastQC (version 0.11.2)