FastQCFastQC Report
Sun 14 Apr 2019
SRR6576478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337775
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5490.16253423136703427No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.10125083265487379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7200.042.442321
GTACATG17700.038.7791141
TACATGG17650.037.8235132
ACATGGG17150.037.5500373
CAACGCA9400.032.9993745
ATCAACG9000.032.9042473
AACGCAG9450.032.824786
TCAACGC9100.032.537854
ATGGGTA2400.031.332745
TCGAGCG300.00416016231.3327454-55
CATGGGA9850.031.0146454
GGTACCT2450.030.6932988
ACGCAGA10900.028.4581767
TGGGTAC2650.028.3768226
CCCTATG1005.302536E-528.2078171
GTATATA1205.814629E-627.4242691
GGGTACC2750.027.3449367
CGCAGAG11500.026.9734028
GTATACA1406.3306106E-726.8645921
ATATTAG1057.3923686E-526.8645881
Produced by FastQC (version 0.11.2)