FastQCFastQC Report
Sun 14 Apr 2019
SRR6576477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375638
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7820.2081791512040848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.1123422018006698No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3980.10595307184044211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9700.042.7009281
GTACATG23000.036.6312331
TACATGG23100.036.2157822
ACATGGG22950.034.809543
CAACGCA13100.032.2851835
AACGCAG13150.032.1624266
ATGGGAG5700.032.1530235
TCAACGC13150.031.8050654
ATCAACG13250.031.2103653
CATGGGA12650.030.4617884
TATCAAC14750.028.35882
ATGGGAC2350.027.9957545
ATGGGTA3050.027.7335035
GGTATAC700.008766483526.8960381
ACGCAGA15650.026.7243847
CGCAGAG16150.026.2054258
GGTCTAA909.4257283E-426.1489261
GGGTACC2900.025.9271057
CATGGGT6050.025.6324794
GGTACCT2950.025.5046398
Produced by FastQC (version 0.11.2)