FastQCFastQC Report
Sun 14 Apr 2019
SRR6576476_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576476_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418603
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7010.16746177165476597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.10200595791238953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9350.043.3414761
TACATGG22500.039.89562
GTACATG22750.039.5611421
ACATGGG23200.037.6789783
ATCAACG12000.034.8564263
AACGCAG12100.034.5683636
CAACGCA12250.034.1450735
TCAACGC12300.034.006274
CATGGGG8850.031.8626674
TATCAAC13800.030.65052
CATGGGT6300.030.5856324
ACGCAGA13650.030.298717
CATGGGA12300.029.4211564
CGCAGAG14200.029.125178
ATGGGTA3900.027.7164345
ATACTAG700.00871944326.9264341
TAGTACT1258.01139E-626.3185624
ATGGGAG6150.025.2181345
TGGGTAC3550.025.1535556
GCAGAGT17150.024.3893389
Produced by FastQC (version 0.11.2)