FastQCFastQC Report
Sun 14 Apr 2019
SRR6576474_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576474_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321372
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6960.21657144990851726No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4660.14500329835828882No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3670.11419787660406008No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3340.10392940268598384No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3300.10268473918076247No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3260.10144007567554111No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3220.10019541217031977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG654.7901267E-850.613961
GTATCAA9500.043.0408941
GTATAGA651.411459E-436.152831
GTACATG21000.035.8085171
TACATGG20950.035.6696432
ACATGGG20700.035.1922453
ATGGGAG4300.033.882775
CTTACTA702.1818752E-433.5704849
CATGGGA9400.032.4990844
ATCAACG12000.032.115763
ATTCGCG452.5899017E-531.33732680-81
AACGCAG12150.031.3324536
TCAACGC12300.031.332454
CAACGCA12400.031.0797735
ATATAGG953.7446807E-529.6833742
GTATACT804.774096E-429.3741724
GGTACCT2750.029.0537268
AGTATAC650.006148508328.9222643
GTAATAC650.006148508328.9222643
TACGTCT650.006148508328.9222644
Produced by FastQC (version 0.11.2)