FastQCFastQC Report
Sun 14 Apr 2019
SRR6576473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463091
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12320.26603842441334424No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA8880.19175496824598187No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7630.16476243330144616No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7300.1576364040760887No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7000.15115819568940014No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5820.1256772427017584No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5790.12502942186308955No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5770.12459754130397697No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5690.12287001906752669No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5490.11855121347640098No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5420.11703963151950697No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5100.11012954257370582No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4970.10732231893947411No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4930.10645855782124897No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT4910.10602667726213638No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4810.10386727446657351No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC4780.10321945362790468No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT4710.10170787167101067No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG4660.10062817027322925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13850.043.4845351
TACACCG450.001014319641.7694744
GTACATG32550.038.73991
ACATGGG32900.037.563963
TACATGG33650.037.0060162
AACGCAG16800.035.8024066
ATCAACG16950.035.208343
TCAACGC17100.035.1742974
CATGGGG9700.034.8796654
CAACGCA17400.034.567845
CGCAGAG19250.031.025088
ACGCAGA19400.031.0041477
ATGGGTA5300.030.1449535
CATGGGA17600.029.9031474
CATGGGT8500.029.852894
CTATACA1952.0008883E-1128.9173284
ATGGGAG7350.028.769795
GGGTACC4750.028.6890347
TATCAAC21400.027.66742
CGGGTAG350.0088413926.85325688-89
Produced by FastQC (version 0.11.2)