FastQCFastQC Report
Sun 14 Apr 2019
SRR6576472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences513096
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13790.26876062179397225No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9410.18339647941126028No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7900.15396728877247143No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7420.14461231426477697No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7420.14461231426477697No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6290.12258914511124624No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6090.11869123906637354No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6030.11752186725291172No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC5810.11323417060355176No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5780.11264948469682087No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5480.10680262562951183No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG5410.10543835851380637No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5200.10134555716669005No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5190.1011506618644464No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.10095576656220279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15200.042.1366461
GTACATG36950.040.9124261
ACATGGG37350.039.8920063
TACATGG37800.039.7901342
ATGGGTA6000.039.1645935
GGGTACC4700.038.997947
GGTACCT4700.037.9979938
CATGGGT9050.037.3902854
CATGGGG11650.033.886624
TCTAAGC1405.0931703E-1033.5729263
ATCAACG19250.031.9858423
GGTATCA7700.031.8036691
CAACGCA19600.031.6513865
AACGCAG19750.031.4116
GTATTAC753.2349437E-431.3959311
TCAACGC19800.031.0943154
ATGGGAT5350.030.7460375
GTACCTG5850.030.52839
GGCCTAT1401.8875653E-830.2126878
GTATGGC1252.359975E-730.078419
Produced by FastQC (version 0.11.2)