FastQCFastQC Report
Sun 14 Apr 2019
SRR6576469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283072
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7460.2635371919511644No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4160.14695907754917475No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3630.12823592584218854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.12682285778883112No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3540.1250565227221343No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3400.12011078453538322No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3190.11269217725525661No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3170.11198564322857787No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3110.10986604114854172No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3100.10951277413520236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6550.042.357531
GTCTTAT757.39775E-637.619231
GGGTACC2300.032.6950387
GTACATG15500.032.4617581
TCAACGC8550.031.8824424
ACATGGG15100.031.7477463
TACATGG15550.031.7357372
CATGGGT3900.031.3327454
CAACGCA8700.031.3327455
ATCAACG8850.030.801683
AACGCAG9250.029.4697156
GTATTTA650.00613110228.9378681
GGTACCT2550.027.646548
CATGGGG7150.026.9505424
CTATAAC700.00882578626.8566382
ACTAGAC700.00882578626.8566383
GCCTAAC350.00883311226.85663812-13
ATGGGTA2900.025.9305465
TATCAAC10700.025.4761562
TGGGTAC3050.024.6552736
Produced by FastQC (version 0.11.2)