FastQCFastQC Report
Sun 14 Apr 2019
SRR6576463_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576463_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438819
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8490.19347384684801705No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5840.1330844835797903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.11941142019830499No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4790.10915662266219102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG405.663849E-447.0151561
CCGCTAA405.6734047E-446.999094
GTATCAA14550.038.7753871
ATGGGTA4850.038.762145
TACATGG29050.035.2815972
GGGTACC4550.035.1201977
GTACATG29200.034.939351
GGTACCT4650.034.3649258
CATGGGT8350.034.3346634
TGGGTAC4700.033.999346
ACATGGG29900.033.952523
ATCAACG18300.030.3054243
AACGCAG18300.030.3054246
TCAACGC18450.030.0590384
CAACGCA18600.029.8166265
CCCGCTA650.00615066228.9225163
CATGGGG10400.028.0186884
GCGTTAC350.0088357426.85662322-23
CGCTAAA700.00883101426.8566235
ACGCAGA20850.026.3735897
Produced by FastQC (version 0.11.2)