FastQCFastQC Report
Sun 14 Apr 2019
SRR6576462_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576462_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253306
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5390.21278611639676914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.19699493892762115No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3840.15159530370382068No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3490.1377780234183162No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3250.1283033169368274No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2890.11409125721459419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.10777478622693501No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2720.1073800067902063No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2690.10619566848002022No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2610.10303743298619061No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2540.10027397692908971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA2150.041.5257265
GTGTATG608.703825E-539.2432441
GTACATG20750.038.5813061
GTATCAA11050.037.929221
TACATGG21100.037.636252
ACATGGG21250.037.591713
ATGGGAT2650.037.237075
CATGGGG8000.034.6548584
CATGGGT4750.034.6239434
CTCGATA550.002705462534.20128694
TAACACT550.002715964334.1742824
ATAAGAC550.002715964334.1742823
CTAGACA702.1830405E-433.564034
ATAACAC851.7520526E-533.1691553
ATGGGGA4400.033.106345
TGTATCG902.5759602E-531.351189
CGGCGTG300.004163295531.32642678-79
TAGTGTC600.00415940231.3264267
AACGCAG12750.031.3264246
GGTACCT1859.094947E-1230.5038498
Produced by FastQC (version 0.11.2)