FastQCFastQC Report
Sun 14 Apr 2019
SRR6576461_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576461_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280117
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6000.2141962108690297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.16493108236915288No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.1502943412931025No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3270.1167369349236212No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3190.11388098544536747No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3010.10745509911929658No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2940.10495614332582458No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2840.1013862064780074No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2810.10031522542366227No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA156.896718E-494.04
CGCTAAA250.005231918856.3999985
CATGGGT4700.042.04
ATGGGTA2600.041.5769235
GGTACCT2350.040.08
GTATCAA11000.038.0272751
GTACATG21100.037.86731
ACATGGG21450.036.5920753
TACATGG21750.036.3034482
CATATAG651.4107625E-436.1538473
GGGTACC2700.034.8148167
TAGGTAT550.002713517834.1818165
AACGCAG12550.032.9561776
CAACGCA12750.032.0705875
ATCAACG12650.031.952573
TCAACGC12750.031.7019624
TAGGGCG600.0041556631.3333344
TGGGTAC3100.030.3225826
CATGGGG7800.028.9230774
GTATTGA856.803135E-427.6470581
Produced by FastQC (version 0.11.2)