FastQCFastQC Report
Sun 14 Apr 2019
SRR6576459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487495
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9130.18728397214330403No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6820.13989887075764879No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6110.12533461881660324No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC5830.11959097016379656No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5070.10400106667760696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTATA250.005235004256.397912
GTATCAA12650.040.5797731
TACATGG27000.034.987592
GTACATG27200.034.8017231
AACGCAG15750.034.3161856
TCAACGC15600.034.043614
CAACGCA15950.033.296575
ATCAACG15750.033.122583
ACATGGG29650.031.8605383
GTATAAT1751.6734703E-1029.6024911
CGCAGAG19200.028.394788
GGTACCT4350.028.0909128
ACGCAGA19250.028.076887
TATCAAC18750.028.0736242
CATGGGG13250.028.0216034
GTATAAG1205.743359E-627.4720081
CATATAG700.00874468626.9113561
TATACCC700.00883271626.856154
GGGTACC4600.026.564237
TAAGGGT1258.013836E-626.3190234
Produced by FastQC (version 0.11.2)