FastQCFastQC Report
Sun 14 Apr 2019
SRR6576457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352147
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6430.18259420071731405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.11302098271460499No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.11018125953082093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13150.037.5863881
TACATGG23000.034.7383462
GTACATG23450.034.526511
ACATGGG23050.034.4590873
AACGCAG15000.032.2726026
GCACCGT753.271398E-431.3326236
ATCAACG15850.030.8384173
TCAACGC15950.030.6450734
CAACGCA15900.030.445855
CATGGGA14300.029.9084134
ATGGGAG5900.027.8807245
ACGCAGA17200.027.8675047
CGCAGAG17700.027.0956768
TATCAAC18400.026.8200442
CATGGGT5200.026.2109454
CCCTATG1101.0165288E-425.635782
GTCATAT950.00129113924.7749861
CCCCTAT950.00129113924.7749861
TCTAGAC950.001302961124.7362823
CATGGGG8600.024.5924664
Produced by FastQC (version 0.11.2)