FastQCFastQC Report
Sun 14 Apr 2019
SRR6576456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379035
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5770.15222868600525016No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4220.11133536480799926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG250.005233631556.3992045
TATCGGT250.005233631556.3992044
GTATCAA10850.040.7183231
TACATGG21750.033.925962
AACGCAG12500.033.8395276
GTACATG22000.033.540441
TCAACGC12900.033.518914
ATCAACG12950.033.3894923
ACATGGG21850.032.9102943
CAACGCA13350.032.389065
AGTGCGC600.004157780731.3328958
TATCAAC14650.030.1565722
TACGACG953.7460737E-529.6837945
ACGCAGA14650.028.8733147
CTACGAC1005.314764E-528.1996024
CGCAGAG15000.027.8862768
CGACGGT1057.4093885E-526.8567667
ACGACGG1057.4093885E-526.8567666
TTGTACG350.008834762526.85676488-89
GTGTAGA1406.350183E-726.8567641
Produced by FastQC (version 0.11.2)