FastQCFastQC Report
Sun 14 Apr 2019
SRR6576455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451135
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10000.22166313852837843No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6130.13587950391789597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.11238321123388786No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4890.10839327474037705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11900.048.649151
ATCAACG15100.037.9644623
ACGATAA250.001702898237.5952190-91
GTACATG28800.036.4442941
TCAACGC15650.036.334
AACGCAG15550.036.2574356
TACATGG29050.035.751042
CAACGCA15800.035.68775
GGGTATA550.002696345634.2305951
ACATGGG29500.034.0867923
TATCAAC18100.032.454432
ATAGTGC902.5809473E-531.3501978
ATGGGCG1201.6519698E-731.325875
CATGGGG11300.030.35564
ACGCAGA18700.029.9052817
CAACGGT650.00615742128.9161854
CGCAGAG19400.028.8454138
ATGGGTA3450.027.2398855
GTGTTAC1057.3401476E-526.8954661
AACGGTC700.00884069826.8507465
Produced by FastQC (version 0.11.2)