FastQCFastQC Report
Sun 14 Apr 2019
SRR6576455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451135
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10160.2252097487448325No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5870.13011626231615814No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5420.12014142108238111No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4570.10130005430746894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG25900.042.828482
GTACATG26150.042.239291
ACATGGG26400.040.7574963
CATGGGT6100.038.513784
GTATCAA9850.037.6973881
ATGGGTA3750.035.0834855
CATGGGG10800.032.6297264
CATGGGA13700.032.2391244
CCGTACA650.006148697328.924582
AACGCAG13150.028.585136
GGGTACC3950.028.5489487
CAACGCA13200.028.4768525
ATCAACG13150.027.8705023
TCAACGC13400.027.7011784
GGTATCA5950.027.6484951
GGTACCT4200.026.8496068
TGGGTAC4350.025.9237586
GTATAAA2501.6370905E-1124.4412691
ACGCAGA15500.023.9481167
ATGGGAT5150.023.7214975
Produced by FastQC (version 0.11.2)