FastQCFastQC Report
Sun 14 Apr 2019
SRR6576453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312505
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.19775683589062573No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3410.10911825410793428No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3310.10591830530711507No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3230.10335834626645975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7150.046.0213551
ACATGGG17000.036.7588233
GTACATG17150.036.4548151
TACATGG17250.036.243482
ATCAACG9150.034.404293
AACGCAG9250.034.032356
CAACGCA9700.032.453535
TCAACGC9800.032.1223684
CATGGGG6900.029.9614264
ACGCAGA10800.029.583127
GACTATC804.7819645E-429.3655997
TATCAAC10850.029.0276872
CCTTATA650.006142596228.927712
CGCAGAG11150.028.6545058
GTATAAT1755.511538E-926.8614431
CATGGGA9450.026.8485474
AGTGCGT350.008846790526.84854732-33
CATGGGC3700.026.667144
ATGGGTA2008.094503E-1025.8417285
CATGGGT3300.025.628164
Produced by FastQC (version 0.11.2)