FastQCFastQC Report
Sun 14 Apr 2019
SRR6576451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474194
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8190.17271412122464644No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.13011552233895832No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5120.10797268628451648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4960.10459853983812532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG30600.037.062081
TACATGG31050.036.6262252
GTATCAA15850.034.7368771
ACATGGG31350.034.626843
CATGGGG10700.032.0610354
CATGGGA18450.030.3102154
GTATAAC804.742275E-429.4112971
GTATAGC1303.2888784E-728.958821
AACGCAG19450.028.2686256
ATCAACG20000.027.2562713
TCAACGC20100.027.1206674
GTATTAT1955.9480953E-1026.5455821
CAACGCA20950.026.2446175
ATGGGAC3650.025.74995
ACGCAGA22000.024.9920337
TGGGTAC3200.024.9653326
ATGGGAG6250.024.8126035
CGCAGAG22600.024.3439318
TATCAAC22600.024.3285272
ATGGGGG4750.023.7441185
Produced by FastQC (version 0.11.2)