FastQCFastQC Report
Sun 14 Apr 2019
SRR6576450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522557
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8010.15328471343795988No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.10869627619570688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG32600.036.9985161
TACATGG33200.035.676662
ATGGGAG8100.034.236455
ACATGGG33650.034.221793
GTATCAA16200.032.2827031
CATGGGA20600.031.487174
CCGCACA650.006149487628.9246674
CTAGTAC1154.1888416E-628.610273
CGACCCG2156.548362E-1126.2340015
ATGGGAT6100.026.198165
AACGCAG20150.026.1255056
ATCAACG20100.025.9566523
GTATAAA2550.025.8672261
CATGGGG11200.025.1799554
CAACGCA21050.025.0085015
TCAACGC21050.025.0085014
GTATATA2351.9463187E-1024.0588761
CATGGGT6500.023.862854
GTAATAG1401.8924768E-523.557651
TAGTACT1602.0342904E-623.5012934
Produced by FastQC (version 0.11.2)