FastQCFastQC Report
Sun 14 Apr 2019
SRR6576449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390264
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8420.2157513888034766No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4620.11838140335772708No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4270.10941311522456594No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4260.10915687842076134No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4210.1078756944017383No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC450.001014471541.766568
GTATCAA10550.040.1045761
TACATGG23800.034.567262
ACATGGG23650.034.371323
GTACATG24250.034.313531
GTATCGG550.002711571834.1901631
AACGCAG14100.029.9919456
TCAACGC14050.029.7642484
ATCAACG13900.029.7474083
CAACGCA14200.029.4498375
CATGGGG9450.028.3415954
ATGGGGA5250.027.744935
CATGGGT6350.027.3784734
TATCAAC15700.026.9492512
ACGCAGA16550.025.2681397
ATGGGTA3550.025.1481765
CATGGGA12950.025.0357484
CGCAGAG16850.024.818268
GTCCTAA950.001301189324.7428821
GTATAAT1452.5229676E-522.6951941
Produced by FastQC (version 0.11.2)