FastQCFastQC Report
Sun 14 Apr 2019
SRR6576448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435815
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8310.19067723690097862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.1255119718229065No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4870.11174466229937013No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840.11105629682319333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG200.002162285470.498289
GTATCAA10950.048.6126481
ATCAACG14250.036.6096343
ACATGGG22050.036.4480933
GTACATG22900.036.4101751
TACATGG22250.036.3316962
AACGCAG14700.036.1283726
CAACGCA14600.035.7320025
TCAACGC14800.035.2491384
CACGATA550.00271549134.1809847
CGCAGAG16100.032.111028
CATGGGA11550.031.7394834
GTATAAT1658.367351E-1131.404631
CATGGGT6300.031.3325714
ACGCAGA16450.031.1420977
TATCAAC17350.030.881092
GTACTAG804.7129163E-429.4418431
CCTATAC650.006150767728.9223693
GCAGAGT18600.028.0477039
ACCGCAC700.00883116526.8564853
Produced by FastQC (version 0.11.2)