FastQCFastQC Report
Sun 14 Apr 2019
SRR6576446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352776
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7680.21770188448193756No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4340.12302424201192826No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4280.12132344603941311No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3870.10970134022722634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAA301.3717936E-462.648715
ACCGAGT450.001014428341.765818
ACATGGG23450.036.2667923
CATGGGT4900.035.479634
GTACATG24100.035.308661
TACATGG24000.035.259892
CCGCTAA550.002718283834.1720284
CCCGCTA550.002718283834.1720283
TGCCCGC1256.2245817E-933.84951
CATGGGG8250.030.185294
ATGGGTA4000.029.3665835
GTATCAA12600.029.1034031
ATGGGAG5500.029.0462235
GTGTAGG1503.6725396E-828.2079141
GGTACCT3200.027.8982548
CATGGGA13350.027.4528084
ATACCAT1205.8411442E-627.408816
TGGGTAC3500.026.8494476
TAAGCTA1057.42171E-526.8494475
TATAGCA1257.9858855E-626.3273872
Produced by FastQC (version 0.11.2)