FastQCFastQC Report
Sun 14 Apr 2019
SRR6576445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390977
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7400.1892694455172555No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4380.11202705018453771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.108446276890968No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4070.10409819503449053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT704.5316647E-640.408431
GTATCAA11850.036.9984361
CATGGGT5850.034.5458834
ACATGGG23200.033.8307883
TACATGG23600.033.655682
GTACATG23600.033.3597831
ATGGGTA3950.032.1255345
CATGGGG7950.031.332314
ATCAACG14300.031.2227553
TCAACGC14400.031.0059324
AACGCAG14000.030.8847056
CAACGCA14600.030.2592855
TATACTG1252.3571192E-730.079025
GGTACCT3500.028.1990788
GGGTACC3700.027.9450347
TATCAAC16650.027.3804872
CTCGATA700.00883042826.85626494
ACGCAGA16400.026.0784197
CGCAGAG16400.026.0784198
TGGGTAC4000.025.8491556
Produced by FastQC (version 0.11.2)