FastQCFastQC Report
Sun 14 Apr 2019
SRR6576445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390977
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8040.20563869485928837No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4710.12046744437652342No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4280.10946935497484506No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4010.10256357790867493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11950.038.557361
GTACATG22400.034.4227031
GCAAACG702.1779138E-433.5831261
GTCTTAC702.1779138E-433.5831261
AACGCAG13900.033.1355176
TACATGG23200.033.033062
ACATGGG23900.032.0532573
TCAACGC14350.031.4413974
ATCAACG14450.031.2238083
CAACGCA14500.031.1161425
GTATAGG1154.175745E-628.6186641
CATGGGA13200.028.1277924
CGCAGAG16400.028.0843728
ACGCAGA16550.027.8298327
CATGGGG8500.027.646084
TATCAAC16750.027.508092
ATGGGAG5150.026.4650865
ACAGCGT909.5083704E-426.1101888
CTTAGGC1101.0142484E-425.6452962
GCAGAGT18350.025.0999329
Produced by FastQC (version 0.11.2)