FastQCFastQC Report
Sun 14 Apr 2019
SRR6576444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378181
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7880.20836583540685544No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.120048336643036No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4420.11687525285511435No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4340.1147598636631666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4260.11264447447121881No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3840.101538681213493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11000.041.4727021
TACATGG20650.036.639072
ACATGGG20350.036.4864163
GTACATG21100.036.109031
AACGCAG13200.033.465136
ATCAACG13250.032.984183
TCAACGC13350.032.7371064
GGTACCT3100.031.8554258
ATGGGTA3250.031.8110435
GGGTACC3250.031.8110437
CAACGCA13750.031.7847565
CATGGGG9750.031.3290584
ACGCAGA14650.030.1528827
CATGGGT5200.029.8228534
CGCAGAG15450.028.3061168
TATCAAC16300.027.38892
TGGGTAC3950.027.3633566
TAGTACT1057.41552E-526.8534774
CATGGGA8950.024.6781964
GCAGAGT17900.024.4318149
Produced by FastQC (version 0.11.2)