FastQCFastQC Report
Sun 14 Apr 2019
SRR6576444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378181
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8720.230577421922307No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5110.13512048463566387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560.12057718394102294No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4270.11290889812021226No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4260.11264447447121881No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4150.10973581433229061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9400.042.9941331
GTACATG20200.038.1532061
ACATGGG20250.038.053973
TACATGG20500.037.8241042
ATCAACG10900.036.2104533
CCCCTAG651.4128308E-436.148911
TCAACGC11400.034.6222764
CAACGCA11950.033.028785
CATGGGG9200.032.686874
AACGCAG11850.032.514476
ATGGGTA2950.031.8558465
TATACTT1252.3616485E-730.0719175
ACGCAGA13250.029.078987
TATCAAC13950.028.6340852
GTGTAGG1704.0636223E-927.6432861
CATGGGA9700.027.611144
CATGGGT4800.027.4093024
CGCAGAG14250.027.038358
CTATAGT700.00884023726.8499284
ATAGGTG909.5207494E-426.1040955
Produced by FastQC (version 0.11.2)