FastQCFastQC Report
Sun 14 Apr 2019
SRR6576443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422197
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7460.17669476571363607No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5020.11890183966252721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.11226986454190815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11150.035.060061
GTACATG23450.034.545781
TACATGG23700.033.9101942
ACATGGG23100.033.77373
CTAATAG600.00415874631.3322263
CATGGGG10100.030.2464564
ATCAACG13750.028.369913
AACGCAG13900.028.0637596
CAACGCA14000.027.86335
TCAACGC13850.027.8257334
ATGGGTA3550.027.8018365
TTATACT1755.5351848E-926.8561944
GATCGTC1606.896698E-826.4365677
CTCGCAA909.503055E-426.11328394
ACGCAGA15700.025.4449637
TATCAAC15350.025.1066072
GTAGTAC950.001287370524.7888181
GGGTACC3800.024.735977
TATACTA950.001303486824.735975
TAACTGC3450.024.5208746
Produced by FastQC (version 0.11.2)