FastQCFastQC Report
Sun 14 Apr 2019
SRR6576438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308889
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8130.2632013441721784No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4630.14989203241293797No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4190.13564743322034778No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3610.11687046155738792No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3480.11266182997775899No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3350.10845319839813006No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3150.10197838058331635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA3350.042.078465
GTATCAA8800.040.5999341
GTACATG19600.035.257831
GGTACCT3250.034.6985478
CATGGGT5150.034.6704754
TACATGG19850.034.3401262
ACCGTGC550.00271745634.172818
ACATGGG19500.033.73473
TAAAGGT902.5924983E-531.3250794
GGGTACC3750.031.3250777
CCTATGC1201.6494596E-731.3250773
AACGCAG11200.030.6258546
ATCAACG11250.030.0720753
TCAACGC11300.029.939014
CAACGCA11550.029.290985
TAGAACC1303.3259494E-728.9154574
TGGGTAC4150.028.3057926
TATCAAC12550.027.7193092
CCAATAC856.815217E-427.6397743
GTACCTG4150.027.173569
Produced by FastQC (version 0.11.2)