FastQCFastQC Report
Sun 14 Apr 2019
SRR6576436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257818
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.3331807709314323No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6800.26375194904932936No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4260.1652328386691387No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4250.16484496815583086No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4170.16174200404936817No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4030.15631181686305845No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3910.15165737070336438No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3770.14622718351705466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.14506357197713116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.13420319760451171No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3370.13071236298474118No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3310.12838513990489417No Hit
GGGCGGGGACGGGCGGTGACTCGCCTCGCGGCGGACCGCCCGCCCGCTCC3250.12605791682504713No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3060.11868837707219822No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3010.11674902450565902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7750.057.208091
CATATAG405.672979E-446.9924353
GGTATCA3100.044.123261
GTGTATA757.2434577E-637.7329941
ATAAGGC500.001700923437.5939523
CTGCTTA757.4293857E-637.593959
ATCAACG11700.036.9513173
GTACATG16300.036.4597971
TCAACGC12000.036.0275344
CAACGCA12150.035.582755
TACATGG16800.035.5309372
ACATGGG16750.034.5078773
AACGCAG12700.034.4117856
ATGGGTA2050.034.384715
TAGTACT550.002715260534.1763154
CATGGGT3950.032.121414
GTCTAGA753.2015407E-431.444161
ACCGAAG300.00415820131.33436822-23
CATGGGG6300.031.3282894
ATGGGGG2750.030.7586865
Produced by FastQC (version 0.11.2)