FastQCFastQC Report
Sun 14 Apr 2019
SRR6576431_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576431_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232781
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3800.16324356369291307No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3110.13360196923288414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.11341131793402381No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2530.10868584635343949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9600.036.3014371
CATGGGT3650.036.049114
ATGGGTA2200.034.176435
CTAGTAC550.002714677234.176433
TAGTACT550.002714677234.176434
AACGCAG10600.033.69286
GTACATG17150.032.4027021
TACATGG17250.031.8800832
ACATGGG17500.031.1493743
CAACGCA11700.030.5250995
TCAACGC11800.030.2664134
TATAATG1102.9537405E-629.91082
ACGCAGA12350.029.2990237
TTACACC650.006148962328.9185164
ATCAACG11700.028.9185143
CGCAGAG12750.028.7669458
TATACAG1057.40455E-526.852915
ATATACG1755.515176E-926.8529076
TATCAAC13200.026.7060722
GTATAAT909.3849516E-426.1631981
Produced by FastQC (version 0.11.2)