FastQCFastQC Report
Sun 14 Apr 2019
SRR6576430_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576430_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253636
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3770.14863820593291174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2630.10369190493463074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7450.046.197881
AACGCAG9000.038.6431476
TACATGG15250.037.9069252
GTACATG15500.036.8051951
ATCAACG9650.035.553213
CAACGCA9850.034.8313145
TCAACGC9850.034.8313144
ATGGGTA2700.034.813655
ACATGGG16200.034.813653
CATGGGT4800.033.290554
CGCAGAG10550.032.0747538
ATATAGA753.2090087E-431.4314331
TATCAAC11000.031.1898652
ACGCAGA11000.030.7626067
CATGGGG5400.029.59164
CTATACT804.7714423E-429.3740144
GTTCCGG650.00614625928.92210694
GCAGAGT12100.027.9660039
GGGTACC2253.6379788E-1227.1546447
GTATAAG700.00868974126.9412291
Produced by FastQC (version 0.11.2)