FastQCFastQC Report
Sun 14 Apr 2019
SRR6576430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253636
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4780.1884590515541958No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3200.1261650554337712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.11788547367093No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2790.1100001577063193No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2780.10960589190808875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6800.038.0361671
GTACATG16400.036.1301421
TACATGG16400.036.1301422
ACATGGG17050.034.1808853
TTACACC804.7712636E-429.37424
CATGGGA10050.028.9942344
CATGGGT4300.028.417834
GTACTGA1005.3084048E-528.1992326
ATCAACG9250.027.9451853
GTCTAGA856.7789335E-427.6626661
AACGCAG9600.026.9263526
AAACGCA700.00882445226.856413
TAGTACA700.00882445226.856414
TCAACGC9850.026.2429394
CATGGGG5200.026.2108254
GTAGGTT909.498681E-426.11046
CAACGCA9950.025.979195
CTACACC1101.01546175E-425.6356644
TATCAAC10400.025.3219812
TTATTGT1501.1568391E-625.0659854
Produced by FastQC (version 0.11.2)